SIH Medium Size:250g 21 pH (100 g/l) 5

21 pH (100 g/l) 5

4 Riboflavin 0

Final concentration of 50mM MES

50 X sterile stock solution

The main difference between SIH and FM is the novel addition of Aspartic acid (1

SIH Medium Size:250g 21 pH (100 g/l) 5SIH medium, developed by Hanh, Friehs and Flaschel (2004), is the next step in the development of synthetic media designed to grow D. discoideum in high cell densities. SIH medium is an improved version of FM medium, as developed by Frank and Kessin (1977). The main difference between SIH and FM is the novel addition of Aspartic acid (1. 1 mM) and increased levels of Lysine (8. 5 mM) and Tryptophane (1. 7 mM). Concentrations of Cysteine, Glutamic