Yeast Nitrogen base without Amino acids, without Ammonium sulphate and without Sodium Size:500g Addition of magnesium and calcium

Addition of magnesium and calcium increases the growth of the recombinant cells

Ammonium Chloride (5 g/l) is replaced by Glutamate (3

With a pKa of 7

chemically and enzymatically stable

Tryptone and Yeast are present in the medium in elevated concentrations and provide nitrogen

Yeast Nitrogen base without Amino acids, without Ammonium sulphate and without Sodium Size:500g Addition of magnesium and calciumSodium chloride, NaCl is omitted from this formulation of Yeast Nitrogen base without Amino acids to provide the researcher the possibility to grow yeast cells in the absence of Sodium or to replace this element. Ammonium sulphate, as a source of Nitrogen, has been omitted to test the ability of yeast cells to assimilate different nitrogen sources like Potassium nitrate. Suspend 1. 8 gram powdered medium in 1 litre distilled water Store dry at room