Yeast Nitrogen base without Amino acids, without Ammonium sulphate and without Biotin Size:100g anh 500 Sodium Chloride 100

anh 500 Sodium Chloride 100 Calcium Chloride

Isopropyl-β-D-1-Thiogalactopyranside C9H18O5S=238

minus Cysteine and without Lysine

2 at 25°C Suspend 16 gram powdered medium in 1 litre distilled water

Soya Peptone 10 Yeast extract 5 NaCl 10 Agar 15 Final pH 7

Yeast Nitrogen base without Amino acids, without Ammonium sulphate and without Biotin Size:100g anh 500 Sodium Chloride 100Biotin is omitted from this formulation of Yeast Nitrogen base without Amino acids to provide the researcher the possibility to grow yeast cells in the absence of biotin or to replace this vitamin. Ammonium sulphate, as a source of Nitrogen, has been omitted to test the ability of yeast cells to assimilate different nitrogen sources like Potassium nitrate. Suspend 1. 9 gram powdered medium in 1 litre distilled water Store dry at room temperature