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To make YEPG-Medium add 38 ml glycerol per litre YEP medium
015 NH4 < 1
2H2SO4=692
05 Total 49
10 Absorbance 280 nm <0
Terrific Broth Size:6 x 1kg To make YEPG-Medium add 38Terrific Broth is rich nutritional medium developed by Tartoff and Hobbs supporting luxurious high density growth of E. coli and a higher yield of plasmid DNA compared to LB type media. Recombinant E. coli cells have an extended growth phase in Terrific medium. Terrific Broth medium is used with glycerol as a carbon source. Unlike glucose, glycerol is not fermented to acetic acid. Tryptone and Yeast are present in the medium in elevated concentrations
