Vegetal LoFlo Medium Size:6 x 1kg allowing for the separation of

allowing for the separation of biomolecules based on their size

Tryptone 8 Yeast extract 5 NaCl 5 Agar 7 Final pH 7

001 Agar 13

used to separate and analyse DNA

6 L-Tyrosine 36

Vegetal LoFlo Medium Size:6 x 1kg allowing for the separation ofLoFlo vs VLoFlo: Triplicate biological repeats (cultures) Cell counts made to 48 h Note: by 26 h cells round and shrink* in both LoFlo and VLoFlo By 47 h cell rounding and shrinkage pronounced and assay stopped * Noted previously in Dicty community that LoFlo is not ideal for long term culturing. Typically, cells are grown only overnight in LoFlo, and transferred to fresh Lo Flo the following morning prior to imaging, so that LoFlo growth limited to