DMEM/F12 is a 1:1 mixture of DMEM (a modified Basal Medium Eagle (BME) formulation with higher concentrations of amino acids and vitamins) and Ham’s F-12 (modified from Ham’s F-10 with increased choline
may require supplementation with growth factors or serum
Defined Bioscience recommends the use of our DMEM/F12 (catalog # LSB-101) when preparing HiDef® B8 complete medium
Standard vials prepare 500 mL of complete medium
for routine aggregate passaging or single cell suspension
Ready-CEPT® Cell Viability Enhancer Size:1 x 200 uL DMEM/F12 is a 1:1 mixtureDefined Biosciences Ready CEPT 1000X cocktail is a defined formulation designed for improved stem cell survival and growth during routine passaging, cryopreservation, and freeze thaw recovery. Ready CEPT is a single vial cocktail of chroman 1 (a ROCK inhibitor), emricasan (a pan caspase inhibitor), polyamines (putrescine, spermine, and spermidine; polycations for cell growth support), and trans ISRIB (an ISR inhibitor). CEPT was originally developed